目的:探讨丹皮酚和阿司匹林和阿司匹林对人结肠癌细胞系LoVo细胞增殖的影响及可能作用机制。方法:用不同浓度丹皮酚(15.63-250mg/L)和阿司匹林(98.08-1 801.6mg/L)处理体外培养的LoVo细胞24,48,72,96h,CKK-8比色法测定其对结肠癌LoVo细胞的活力的影响;根据CKK-8结果分为丹皮酚组(浓度31.25,62.50,125mg/L)和阿司匹林组(浓度分别为180.16,900.80,1 801.6mg/L),进一步处理48h后行流式细胞仪检测细胞凋亡、激光共聚焦显微镜检测细胞内Ca2+浓度的变化及RT-PCR法检测RUNX3基因表达情况。结果:丹皮酚(浓度15.63-250mg/L)和阿司匹林(浓度90.08-1 801.6mg/L)均能能显著降低LoVo细胞存活率,呈剂量、时间依赖性;丹皮酚组处理细胞48h后可诱导细胞凋亡,流式细胞仪检测凋亡率分别为13.5%,21.4%,34.6%,明显高于对照组;阿司匹林组细胞凋亡率分别为14.8%,25.8%,37.7%,明显高于对照组;丹皮酚组荧光强度分别为41.36±4.62,57.51±3.83和69.43±3.76;阿司匹林组荧光强度分别为38.24±4.62,53.31±4.92和65.64±5.25,丹皮酚组、阿司匹林组与对照组(荧光强度24.45±3.74)比较,差异均有统计学意义(P<0.05);丹皮酚和阿司匹林均能上调RUNX3mRNA的表达,呈剂量依赖性。结论:丹皮酚、阿司匹林均能抑制LoVo细胞的增殖并诱导其凋亡,其作用机制可能与增加细胞内Ca2+含量和上调RUNX3基因的表达有关。丹皮酚与阿司匹林可能具有相似抗大肠癌细胞作用机制。 Objective: To investigate the effects of paeonol, aspirin and aspirin on the proliferation of human colon cancer cell line LoVo and its possible mechanism. Methods: LoVo cells cultured in vitro were treated with different concentrations of paeonol (15.63-250mg / L) and aspirin (98.08-1801.6mg / L) for 24, 48, 72, 96h and CKK-8 colorimetric assay According to the results of CKK-8, the cells were divided into paeonol group (31.25,62.50,125mg / L) and aspirin group (180.16,900.80,1801.6mg / L respectively) for 48h Apoptosis was detected by flow cytometry. The changes of intracellular Ca2 + concentration were detected by laser scanning confocal microscopy and the expression of RUNX3 gene was detected by RT-PCR. Results: Paeonol (concentration 15.63-250mg / L) and aspirin (concentration 90.08-1 801.6mg / L) could significantly reduce the survival rate of LoVo cells in a dose-and time-dependent manner; Paeonol treated cells 48h The apoptotic rate of the aspirin group was 14.8%, 25.8%, 37.7% respectively, which was significantly higher than that of the control group (13.5%, 21.4%, 34.6%, respectively) In the control group, the fluorescence intensity of paeonol group was 41.36 ± 4.62, 57.51 ± 3.83 and 69.43 ± 3.76, respectively. The fluorescence intensity of aspirin group was 38.24 ± 4.62, 53.31 ± 4.92 and 65.64 ± 5.25, respectively. Paeonol group, aspirin group and Compared with control group (fluorescence intensity 24.45 ± 3.74), the difference was statistically significant (P <0.05). Both paeonol and aspirin up-regulated RUNX3 mRNA expression in a dose-dependent manner. CONCLUSION: Both paeonol and aspirin can inhibit the proliferation of LoVo cells and induce their apoptosis. The mechanism may be related to the increase of intracellular Ca2 + content and the up-regulation of RUNX3 gene expression. Paeonol and aspirin may have a similar mechanism of action against colorectal cancer cells.