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目的:建立荭草花HPLC特征指纹图谱,为该药材的品质评价提供参考。方法:以花旗松素为参照物,采用HPLC法,Diamonsil C18(200 mm×4.6 mm,5μm)色谱柱;流动相为乙腈-0.1%磷酸(梯度洗脱);检测波长为280 nm;流速为1.0 mL/min;柱温为30℃。结果:12个不同产地的荭草花药材指纹图谱标定出18个共有峰,其中指认出4个共有峰,分别为花旗松素、儿茶素、没食子酸、3,3’-二甲氧基鞣花酸-4-O-β-D-葡萄糖苷。通过“中药色谱指纹图谱相似度评价系统”软件计算,12个不同产地荭草花指纹图谱相似度均大于0.9。结论:本实验方法重现性好,指纹图谱专属性强,可为荭草花药材的鉴别及质量控制提供科学依据。
OBJECTIVE: To establish a fingerprint chromatogram of Rhizoma Polygoni cuspidati to provide a reference for the quality evaluation of Rhizoma Chuanxiong. Methods: Taxifolin was used as the reference substance, and Diamonsil C18 (200 mm × 4.6 mm, 5 μm) column was used as mobile phase. The mobile phase was acetonitrile - 0.1% phosphoric acid (gradient elution). The detection wavelength was 280 nm. 1.0 mL / min; column temperature was 30 ℃. Results: The fingerprints of 18 medicinal herbs from different regions were identified as 18 common peaks, of which 4 common peaks were identified, which were Taxifolin, catechin, gallic acid, 3,3’-dimethoxy tannin Sulphate -4-O-β-D-glucoside. Through the similarity evaluation system of chromatographic fingerprints of Chinese traditional medicine, the similarity of fingerprints of the flowers of Phalaris arundinacea in 12 different habitats were all greater than 0.9. Conclusion: The experimental method has good reproducibility and strong fingerprint specificity, which can provide a scientific basis for the identification and quality control of Cymbidium.