论文部分内容阅读
目的:观察PeroxiredoxinⅡmRNA在小鼠卵巢各级卵泡和MII卵中的分布,为了解PeroxiredoxinⅡ在卵母细胞发育及成熟过程 中的功能意义提供形态学依据.方法:原位杂交方法.结果:在小鼠卵巢内,原始卵泡的初级卵母细胞未检到PeroxiredoxinⅡ mRNA的杂交信号,初级卵泡的初级卵母细胞可检到杂交信号,次级卵泡和近成熟卵泡中卵母细胞的信号明显增强.离开卵巢的 GV卵也能检到较强的杂交信号,排到输卵管的次级卵母细胞(MII卵)杂交信号与GV卵相比明显减弱.从原始卵泡到近成熟卵 泡周围的卵泡细胞均可检到杂交信号,其信号强度在卵泡发育成熟过程中未见有明显变化.卵母细胞和卵泡细胞的Peroxiredoxin ⅡmRNA杂交信号均分布在胞质.结论:提示PeroxiredoxinⅡ可能参与了卵母细胞生长发育和卵母细胞成熟过程的调节.
OBJECTIVE: To observe the distribution of peroxiredoxin Ⅱ mRNA in ovarian follicles and MII eggs of mice, and to provide a morphological basis for understanding the functional significance of Peroxiredoxin Ⅱ during oocyte development and maturation.Methods: In situ hybridization was performed in mice Ovarian primordial follicles of primary oocytes were not detected Peroxiredoxin Ⅱ mRNA hybridization signal, the primary follicle of primary oocytes can detect hybridization signal, secondary follicles and nearly mature follicles in the oocyte signal was significantly increased. Of GV eggs also detected a stronger hybridization signal, and the hybridization signal to secondary fallopian tube (MII egg) was significantly weaker than that of GV eggs.From the primordial follicle to the follicle around the mature follicle To the hybridization signal, the signal intensity did not change obviously in the process of follicular development.The Peroxiredoxin ⅡmRNA of oocytes and follicle cells were all distributed in the cytoplasm.Conclusion: Peroxiredoxin Ⅱ may be involved in the oocyte growth and development Regulation of blast maturation.