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采用聚丙烯酰胺凝胶垂直梯度凝胶电泳法,对点带石斑鱼与褐点石斑鱼7种组织中的4种同工酶(EST、LDH、MDH、SOD)的组织特异性进行比较研究。试验结果表明:2种石斑鱼的4种同工酶均具有明显的组织特异性。2种石斑鱼同一组织间MDH酶谱没有差别;LDH酶谱相似,只在迁移率上略有差异;EST、SOD酶谱间存在不同程度的种间差异,可作为鉴定点带石斑鱼与褐点石斑鱼的遗传标记。
The tissue specificity of four isozymes (EST, LDH, MDH, SOD) in seven tissues of spotted grouper and spotted grouper was compared using polyacrylamide gel vertical gradient gel electrophoresis the study. The experimental results showed that the four isozymes of two species of grouper had obvious tissue specificity. There was no difference in the MDH zymogram between the two species of grouper; LDH zymogram was similar, only a slight difference in mobility; different between the EST, SOD zymogram species interspecies differences, can be used as identification point with grouper Genetic markers with brown spots grouper.