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通过Northernblot和NADPH－心肌黄酶染色显示一氧化氮合成酶活性的组织化学分析方法，观察了脂多糖对大氧心脏、主动脉和肾组织中诱导型一氧化氮合成酶基因表达的影响及诱导型一氧化氮合成酶被脂多糖诱导表达的动力学。结果表明，未经脂多糖处理的大鼠诱导型一氧化氮合成酶基因在所检测的三种组织中表达活性很低，脂多糖作用于大鼠2h，心、肾和主动脉中的诱导型一氧化氮合成酶mRNA开始增加，6h达到峰值，此后，逐渐下降，24h回复到对照水平。一氧化氮合成酶组织化学染色显示，对照大鼠的组织细胞内存在较低的组成型一氧化氮合成酶活性，被脂多糖处理不同时间后，三种组织中的一氧化氮合成酶活性均显著升高，到24h仍维持在较高水平，提示诱导型一氧化氮合成酶被合成后，在组织细胞内较为稳定。
Histochemical analysis of nitric oxide synthase activity by Northern blot and NADPH-diaphorase staining was performed to investigate the effect and induction of lipopolysaccharide on gene expression of inducible nitric oxide synthase in the heart, aorta and kidney of the rats Kinetics of Nitric Oxide Synthase Induced by Lipopolysaccharide. The results showed that non-lipopolysaccharide-induced rat inducible nitric oxide synthase gene expression in the three tested in the low activity of lipopolysaccharide in rats 2h, heart, kidney and aortic inducible Nitric oxide synthase mRNA began to increase, reaching the peak 6h, then gradually decreased, returned to the control level 24h. Nitric oxide synthase histochemical staining showed that there was a lower level of constitutive nitric oxide synthase activity in the control rat tissue cells. After being treated with lipopolysaccharide for a long time, the activities of nitric oxide synthase Significantly increased to 24 hours remained at a high level, suggesting that after inducible nitric oxide synthase was synthesized, more stable in the tissue cells.