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目的:构建新型的具有可控性且能诱发动物血栓的栓塞剂——磁靶向凝血蛋白,从而为动物血栓模型的建立提供新工具。方法:使用化学共沉淀法合成磁流体(magnetic nanoparticles,MNPs),通过透析对其纯化后用超导量子干涉磁强针(SQUID)测定其磁性能;利用基因工程方法表达截短组织因子(truncated tissue factor,tTF)(又称为凝血蛋白)并纯化与鉴定;采用戊二醛交联法构建磁靶向凝血蛋白(MNPs-tTF),体外分析其磁控靶向性及凝血效力。结果:成功构建了磁靶向凝血蛋白MNPs-tTF,体外实验证实其保留有磁流体MNPs的磁控靶向性,以及凝血蛋白tTF的凝血效力。结论:成功构建了磁靶向凝血蛋白MNPs-tTF,为血栓模型动物的建立提供一种新工具。
OBJECTIVE: To construct a new type of embolic agent that can be controlled and can induce animal thrombosis, a magnetic targeting coagulation protein, which can provide a new tool for the establishment of animal thrombosis model. Methods: Magnetic nanoparticles (MNPs) were synthesized by chemical coprecipitation. Their magnetic properties were determined by SQUID after purification by dialysis. The truncated tissue factor tissue factor, tTF) (also known as coagulation protein) were purified and identified. Magnetic targeting coagulation proteins (MNPs-tTF) were constructed by glutaraldehyde cross-linking, and their magnetic targeting and coagulation potency were analyzed in vitro. Results: Magnetic targeting coagulation proteins MNPs-tTF were constructed successfully. In vitro experiments confirmed that they retained the magnetic targeting of MNPs and the coagulation efficiency of coagulation protein tTF. Conclusion: Magnetic targeting coagulation protein MNPs-tTF has been successfully constructed, which provides a new tool for the establishment of thrombus model animals.