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作者对乙型肝炎表面抗原(HBsAg)、乙型肝炎e抗原(HBeAg)阳性的慢性乙型肝炎(CHB),伴有或不伴有急性发作的病人14例,检测其外周血单核细胞(PBMC)和血清中2′,5′-寡腺苷酸合成酶(2-5AS)活性,以确定CHB急性发作时干扰素(IFN)系统的体内状态。 14例CHB病人中7例伴急性发作。所有病人血清HBsAg、HBeAg阳性,具CHB的组织学特点。10名健康成年志愿者作对照。病人从未经皮质类固醇或抗病毒药物如IFN、阿糖腺苷等治疗。测定HBsAg、抗-HBs、HBeAg、抗-HBe、乙肝型炎核心抗体(抗-HBc)、抗-HAVIgM、抗-Delta、抗-HBcIgM、DNA多聚酶(DNA-P)、HBV DNA等。还测定血清谷丙转氨酶(ALT)(正常值≤40KU)。CHB急性发作诊断根据为临床症、伴随ALT突然升高达正常值5倍以上。并测定PBMC和血清中2-5AS活性及血清IFN。结果表明,不伴有急性发作的CHB病人7例随访9～21周,5例ALT波动于40～70KU,2例波动于200～300KU,平均为118±105KU。所有病人
The authors examined 14 patients with hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) -positive chronic hepatitis B (CHB), with or without acute exacerbations and examined their peripheral blood mononuclear cells ( PBMC) and 2 ’, 5’-oligoadenylate synthase (2-5AS) activity in serum to determine the in vivo status of the interferon (IFN) system at the onset of CHB. Seven of 14 CHB patients with acute exacerbations. All patients with serum HBsAg, HBeAg-positive, with CHB histological features. 10 healthy adult volunteers as a control. Patients have never been treated with corticosteroids or antiviral drugs such as IFN, vidarabine and the like. HBsAg, anti-HBe, hepatitis B core antibody (anti-HBc), anti-HAVIgM, anti-Delta, anti-HBcIgM, DNA-P, HBV DNA and the like. Serum alanine aminotransferase (ALT) was also measured (normal ≤ 40 ku). The diagnosis of acute exacerbation of CHB is based on clinical manifestations with a sudden increase in ALT of more than 5 times normal. The activity of 2-5AS and serum IFN in PBMC and serum were measured. The results showed that seven patients with CHB without acute attack were followed up for 9-21 weeks. Five patients had ALT fluctuated from 40-70 KU and two patients fluctuated from 200-300 KU with an average of 118 ± 105 KU. All patients