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目的了解河南省2010年柯萨奇病毒A组16型(CA16)流行株VP1区基因特征。方法对河南省2010年手足口病(HFMD)粪便和咽拭子样品中分离到的10株CA16病毒,采用反转录-聚合酶链反应(RT-PCR)方法进行VP1编码区基因扩增,对扩增产物进行测序,利用生物信息学软件对序列分析,与已报道的CA16标准株序列构建基因亲缘关系树。结果 10株CA16毒株,其VP1区核苷酸和氨基酸同源性分别为90.9%~100.0%,99.3%~100.0%,与国际CA16标准株G10在VP1区核苷酸和氨基酸同源性分别为67.0%~69.0%,72.0%~74.0%。亲缘进化树显示全部CA16株可分为A和B两个基因型,B基因型又可分为B1和B2两个基因亚型。河南省分离的CA16毒株全部属于B1基因亚型,同时包含B1a和B1b两条进化分支在河南省共同流行。结论河南省手足口病感染CA16病毒的流行株属B1基因亚型,有B1a和B1b两个进化分支共同进化和循环。
Objective To investigate the genotypes of VP1 in Coxsackievirus A group 16 (CA16) strains in Henan Province in 2010. Methods Ten CA16 viruses isolated from feces and throat swab samples from HFMD in Henan province were amplified by RT - The amplified product was sequenced. The bioinformatics software was used to sequence analysis and construct the genetic relationship tree with reported CA16 standard strain sequence. Results The nucleotide and amino acid identities of VP1 in 10 strains of CA16 were 90.9% -100.0% and 99.3% -100.0%, respectively. The nucleotide and amino acid homology of CA16 with G10 in VP1 67.0% -69.0%, 72.0% -74.0%. Phylogenetic tree shows that all CA16 strains can be divided into A and B two genotypes, B genotype can be divided into B1 and B2 two gene subtypes. CA16 strains isolated in Henan Province all belonged to the B1 gene subtype, while the two evolutionary branches B1a and B1b were endemic in Henan Province. Conclusion The epidemic strain of CA16 virus infected by hand-foot-mouth disease in Henan Province belongs to the B1 gene subtype. There are two evolutionary branches of B1a and B1b that evolve and circulate together.