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目的 检测肿瘤 睾丸抗原SSX1基因mRNA在肝细胞肝癌 (HCC)中的表达情况。方法 用逆转录 聚合酶链反应 (RT PCR)对 47例HCC患者的癌组织和相应癌旁组织以及 1 5例肝硬化和 1 5例正常肝组织的SSX1基因mRNA表达情况进行检测 ,随机选择 4例RT PCR扩增产物的目的片段进行DNA序列测定。结果 47例HCC患者中 ,39例表达SSX1mRNA ,阳性率为83 % ,相应的癌旁组织中有 3例为弱阳性 ,其中 2例患者获得了离癌灶更远处的活检组织 ,对其进行RT PCR检测 ,结果显示阴性 ;所测的 1 5例肝硬化和 1 5例正常肝组织均未检测到SSX1的表达。4例DNA测序结果表明RT PCR产物确为SSX1cDNA。SSX1的表达与年龄、性别、肿瘤大小、分化程度、血清甲胎蛋白 (AFP)水平、乙型肝炎病毒 (HBV)和丙型肝炎病毒 (HCV)感染无显著相关性(P >0 .0 5)。结论 SSX1在HCC中呈高比例、高特异表达 ,可望成为HCC免疫治疗的理想靶位
Objective To detect the expression of tumor testis antigen SSX1 mRNA in hepatocellular carcinoma (HCC). Methods Reverse transcription polymerase chain reaction (RT PCR) was used to detect the mRNA expression of SSX1 in 47 cases of HCC and corresponding adjacent tissues, as well as in 15 cases of cirrhosis and 15 normal liver tissues. Example RT PCR amplification of the target fragment for DNA sequencing. Results Of the 47 HCC patients, 39 cases expressed SSX1 mRNA with a positive rate of 83%. Three of the corresponding paracancerous tissues were weakly positive, and 2 of them obtained biopsy tissues farther away from the foci. The results of RT PCR showed negative results. No SSX1 expression was detected in 15 cirrhotics and 15 normal liver tissues. Four DNA sequencing results showed that the RT PCR product was indeed SSX1 cDNA. There was no significant correlation between the expression of SSX1 and age, sex, tumor size, differentiation degree, serum AFP level, hepatitis B virus (HBV) and hepatitis C virus (HCV) infection (P> 0.05) ). Conclusion SSX1 is highly expressed and highly expressed in HCC, which is expected to be the ideal target for immunotherapy of HCC