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利用差异蛋白质组的方法识别心功能衰竭大鼠心肌代谢的异常 ,以期发现新的治疗靶点。对大鼠行冠状动脉左前降支结扎 ,术后 4周 ,左心室射血分数达 4 6 .4 %± 10 .9% ,成功建立心功能衰竭大鼠模型。实验动物分心功能衰竭组 (n =8)和对照组 (n =8) ,分别取左心室进行线粒体抽提。利用双向凝胶电泳技术显示差异蛋白的表达谱。再经胶内酶解 ,行质谱鉴定和蛋白数据库分析。结果发现 ,双向电泳显示 3个蛋白位点表达有显著差异 ,其中一条蛋白被证实为乙醛脱氢酶 2 ,它在心功能衰竭心肌中的表达显著下降。在心肌梗死后 3、7、14及 2 8天心肌乙醛脱氢酶 2mRNA的表达呈逐渐下降趋势。结果提示 ,在心功能衰竭大鼠的心肌线粒体中乙醛脱氢酶 2表达显著下调 ,而乙醛脱氢酶与硝酸甘油的转化有关。这为临床心功能衰竭患者使用硝酸甘油提供了很有价值的依据。
Differential proteomic methods were used to identify myocardial metabolic abnormalities in rats with heart failure and to find novel therapeutic targets. Left anterior descending coronary artery ligation was performed in rats. After 4 weeks, left ventricular ejection fraction reached 46.4% ± 10.9%, and a rat model of heart failure was successfully established. The rats in the experimental group were divided into two groups: n = 8 in distraction failure group and n = 8 in control group. Mitochondria were extracted from the left ventricle. Two-dimensional gel electrophoresis technique was used to show the differential expression profiles. Then by gel in solution, line mass spectrometry identification and protein database analysis. The results showed that two-dimensional electrophoresis showed significant differences in the expression of three protein spots, of which one protein was confirmed as acetaldehyde dehydrogenase 2, its expression in cardiac failure significantly decreased. At 3, 7, 14 and 28 days after myocardial infarction, the expression of myocardial ALDH 2 mRNA gradually decreased. The results suggest that the expression of ALDH2 is significantly down-regulated in myocardial mitochondria of rats with heart failure, while ALDH is associated with the conversion of nitroglycerin. This provides a valuable basis for the use of nitroglycerin in patients with clinical heart failure.