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提要研究病毒和促癌物在食管癌形成的作用，用人胚食管上度培养和HPV18E6E7AAV感染。培养细胞分二组，SHEEC1组培养基中加入TPA(5ng/mL)4周。对照组SHEE未加TPA。细胞转化的形态，细胞周期,软琼脂培养和裸鼠致瘤性等指标皆证明SHEEC1已恶性转化。E6E7基因在FISH和PCR检测，两组
Abstract To study the role of viruses and carcinogens in the formation of esophageal carcinomas, human embryo esophageal cultures and HPV18E6E7AAV infection were used. The cultured cells were divided into two groups. TPA (5ng / mL) was added to SHEEC1 group for 4 weeks. SHEE did not add TPA in the control group. Morphology of cell transformation, cell cycle, soft agar culture and tumorigenicity of nude mice all proved that SHEEC1 had been transformed malignantly. E6E7 gene was detected by FISH and PCR in two groups