目的:对巫溪款冬花不同产地样品中芦丁含量进行测定。方法:采用HPLC法,迪马C18柱(250mm×4.6mm×5μm),甲醇-0.025mol/L磷酸水溶液(45:55)为流动相,检测波长360nm,外标法定量。结果:芦丁在0.02μg～0.50μg范围呈良好的线性关系,平均加样回收率在98.21%(n=6),RSD为1.88%。结论:此方法准确可行。 Objective: To determine the content of rutin in samples of different origins of Coltsfoot from Wuxi. METHODS: The HPLC method was used, and a Dima C18 column (250 mm×4.6 mm×5 μm), methanol-0.025 mol/L phosphoric acid aqueous solution (45:55) was used as the mobile phase, the detection wavelength was 360 nm, and the external standard method was used for quantification. RESULTS: A good linear relationship was found between 0.02 μg and 0.50 μg for rutin. The average recovery was 98.21% (n=6) and the RSD was 1.88%. Conclusion: This method is accurate and feasible.