【摘 要】
：
利用Illumina MiSeq测序和定量PCR技术测定宁夏中卫市兴仁镇压砂西瓜连作10 a的土壤微生物群落结构变化,结合土壤理化性质分析压砂西瓜连作下土壤生态系统响应.结果表明,随连作年限的增加,土壤pH、养分含量和土壤酶活性都逐渐下降,土壤容重呈增加趋势;连作导致压砂西瓜土壤细菌群落多样性和丰富度显著降低,真菌多样性和丰富度显著增加;细菌和真菌主要优势物种组成发生了变化,细菌中变形菌门相对丰度减少,占真菌群落总丰度的80％以上的子囊菌门和担子菌门的相对丰度随连作年限延长有差异;细菌群落生物量显著下降,
【机 构】
：
兰州大学学报医学版编辑部,兰州730000;中国科学院西北生态环境资源研究院,甘肃省极端环境微生物资源与工程重点实验室,兰州730000;兰州大学草地农业科技学院,兰州730000
论文部分内容阅读
利用Illumina MiSeq测序和定量PCR技术测定宁夏中卫市兴仁镇压砂西瓜连作10 a的土壤微生物群落结构变化,结合土壤理化性质分析压砂西瓜连作下土壤生态系统响应.结果表明,随连作年限的增加,土壤pH、养分含量和土壤酶活性都逐渐下降,土壤容重呈增加趋势;连作导致压砂西瓜土壤细菌群落多样性和丰富度显著降低,真菌多样性和丰富度显著增加;细菌和真菌主要优势物种组成发生了变化,细菌中变形菌门相对丰度减少,占真菌群落总丰度的80％以上的子囊菌门和担子菌门的相对丰度随连作年限延长有差异;细菌群落生物量显著下降,真菌群落生物量逐渐增加,细菌与真菌生物量比值随连作年限的延长持续显著减小.土壤微生物群落的变化可灵敏反映连作压砂西瓜的土壤质量问题.
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