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肿瘤细胞对化疗药物产生抗药性的原因有几种，其中最主要的是多药抗性基因（MDRI）过量表达。定量检测分析MDRI表达水平可说明病人的抗药能力的高低,判断预后,指导临床化疗。本研究通过逆转录PCR方法定量检测了20例大肠癌病人癌组织中MDRI的表达情况．其中包括10例未化疗过的病人．10倒多次化疗的病人．结果显示未化疗的病人MDR1的扩增量（平均为0．056）明显低于化疗后的病人（0.36），两者差异显著，表明MDR1扩增量与化疗密切相关．对临床化疗及判断预后有重要的参考价值。
There are several reasons why tumor cells develop resistance to chemotherapeutic agents, the most important of which is overexpression of the multidrug resistance gene (MDRI). Quantitative detection and analysis of MDRI expression levels can indicate the level of resistance of patients, determine the prognosis, and guide clinical chemotherapy. In this study, the expression of MDRI in 20 cases of colorectal cancer was quantitatively detected by reverse transcription-PCR. Including 10 untreated patients. 10 patients with multiple chemotherapy. The results showed that the amount of MDR1 amplification in patients without chemotherapy (average 0.056) was significantly lower than that in patients after chemotherapy (0.36), the difference between the two was significant, indicating that MDR1 amplification is closely related to chemotherapy. It has important reference value for clinical chemotherapy and prognosis.