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AIM:To study the effect of selenium on peripheral bloodmononuclear cell(PBMC)membrane fluidity and immunefunction in patients with chronic hepatitis.METHODS:PBMCs were pretreated with selenium(1.156×10~(-7)mol/L)for 6 h in v/boor extracted directly frompatients after administration of selenium-yeast continuouslyfor 8-12 wk(200 μg/d),and then exposed to Con-A for48 h.The membrane fluidity,interleukJn-2(IL-2)productionand interleukin-2 receptor(IL-2R)expression in PBMCs andmalondialdehyde(MDA)concentration in medium and lipidperoxide(LPO)in plasma were determined.RESULTS:The PBMC membrane fluidity,IL-2 productionand IL-2R expression in patients with chronic hepatitis weresignificantly lower than those in healthy blood donators(particle adhesive degree R,0.17±0.01 vs 0.14±0.01,P<0.01;IL-2,40.26+9.55 vs72.96±11.36,P<0.01;IL-2R,31.05±5.09 vs 60.58±10.56,P<0.01),and the MDAconcentration in medium in patients with chronic hepatitiswas significantly higher than that in healthy blood donators(1.44±0.08 vs0.93±0.08,P<0.01).Both in vib-o and in vivoadministration of selenium could reverse the above parameters.CONCLUSION:Supplement of selenium can suppress lipidperoxidation,and improve PBMC membrane fluidity andimmune function in patients with chronic hepatitis.
AIM: To study the effect of selenium on peripheral blood mononuclear cell (PBMC) membrane fluidity and immunefunction in patients with chronic hepatitis. METHODS: PBMCs were pretreated with selenium (1.156 × 10 -7 mol / L) for 6 h in v / boor extracted directly from patients after administration of selenium-yeast continuously for 8-12 wk (200 μg / d) and then exposed to Con-A for 48 h. The membrane fluidity, interleukJn-2 (IL-2) production and interleukin-2 receptor RESULTS: The PBMC membrane fluidity, IL-2 production and IL-2R expression in patients with chronic hepatitis weignificantly lower than those in (IL-2R) expression in PBMCs andmalondialdehyde (MDA) concentration in medium and lipidperoxide (LPO) in plasma were determined. P <0.01; IL-2R, 31.05 ± 5.09 vs 60.58 ± 10.56, P <0.01; P < P <0.01), and the MDA concentration in medium in patients with chronic hepatitis was significantly higher than that in healthy blood don ators (1.44 ± 0.08 vs 0.93 ± 0.08, P <0.01) .Both in vib-o and in vivo administration of selenium could reverse the above parameters. CONCLUSION: Supplement of selenium can suppress lipidperoxidation, and improve PBMC membrane fluidity andimmune function in patients with chronic hepatitis.