[Objective]To study the technology condition of surfactant-assisted extraction of total flavonoids from FOLIUM MORI. [Method]Based on the results of single factor test,orthogonal test was used to optimize the extraction condition. [Result]The optimal extraction conditions were as follows: 1.5% Tween 80 solution,1∶40( g/ml) solid-liquid ratio,120 min extraction time,80 ℃ extraction temperature,and 2 extraction times. Under this condition,the average extraction rate of total flavonoids was 1.742%. [Conclusion]Surfactant-assisted extraction was a new and efficient extraction method,which could be used for the extraction of total flavonoids from FOLIUM MORI. [Objective] To study the technology condition of surfactant-assisted extraction of total flavonoids from FOLIUM MORI. [Method] Based on the results of single factor test, orthogonal test was used to optimize the extraction condition. [Result] The optimal extraction conditions were as follows: 1.5% Tween 80 solution, 1:40 (g / ml) solid-liquid ratio, 120 min extraction time, 80 ° C extraction temperature, and 2 extraction times. %. [Conclusion] Surfactant-assisted extraction was a new and efficient extraction method, which could be used for the extraction of total flavonoids from FOLIUM MORI.