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本试验研究了氮离子注入水稻干种子引发的生物学效应,并将其与γ射线、叠氮化钠处理进行了比较。结果表明:氮离子注入后,种胚表层细胞出现刻蚀,细胞之间出现裂缝,种胚内层结构也发生变化。氮离子注入的M1代生理损伤较轻,M2代的叶绿素突变频率低于γ射线和叠氮化钠的处理,抽穗期突变频率高于γ射线和叠氮化钠的处理,株高突变频率与叠氮化钠处理的相近,但比γ射线处理的低。用诱变效率衡量其诱变效果,氮离子注入的叶绿素诱变效率低于γ射线和叠氮化钠的处理,株高的诱变效率则高于γ射线和叠氮化钠的处理,抽穗期诱变效率高于γ射线的处理,但比叠氮化钠处理的略低。
This study investigated the biological effects of nitrogen ion implantation on dry seeds of rice and compared them with γ-ray and sodium azide treatment. The results showed that after the implantation of nitrogen ions, the surface cells of seed embryos were etched, the cracks appeared between the cells and the inner layer structure of seed embryo also changed. The physiological damage of M1 generation in nitrogen ion implantation was lighter, the frequency of M2 chlorophyll mutation was lower than that of γ-ray and sodium azide, the mutation frequency at heading stage was higher than γ-ray and sodium azide, Sodium azide treatment is similar but lower than gamma-ray treatment. The mutagenic efficiency was measured by mutagenic efficiency. The chlorophyll mutagenesis efficiency of nitrogen ion implantation was lower than that of γ-ray and sodium azide. The mutagenic efficiency of plant height was higher than that of γ-ray and sodium azide treatment. The efficiency of phase mutagenesis was higher than that of γ-rays but slightly lower than sodium azide treatment.