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目的检测水通道蛋白5(AQP5)在人大肠癌组织中的表达情况,分析其在大肠癌发病中的临床意义。方法采用逆转录聚合酶链反应(RT-PCR),免疫印迹(Westernblot),免疫组织化学的方法检测水通道蛋白5在人大肠癌组织和癌旁正常组织中的表达情况。结果AQP5在人大肠癌组织表达丰富,而在癌旁正常组织几乎没有表达,两者差异有统计学意义(P<0.05)。结论AQP5在人大肠癌组织中高表达,其在人大肠癌的发生、发展及转移过程中可能起重要作用。
Objective To detect the expression of aquaporin 5 (AQP5) in human colorectal cancer and to analyze its clinical significance in the pathogenesis of colorectal cancer. Methods RT-PCR, Western blot and immunohistochemistry were used to detect the expression of aquaporin-5 in colorectal cancer tissues and adjacent normal tissues. Results AQP5 was abundantly expressed in human colorectal cancer tissues, while almost normal tissues were absent. The difference was statistically significant (P <0.05). Conclusion AQP5 is overexpressed in human colorectal cancer and may play an important role in the carcinogenesis, development and metastasis of human colorectal cancer.