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目的:研究A群溶血性链球菌制剂对NOD小鼠(自身免疫性糖尿病的动物模型)糖尿病的预防作用。方法:用制剂0.1mg皮下注射5周龄♀NOD小鼠,每周一次,共16周,观察其对小鼠胰岛β细胞损伤程度和糖尿病发病率的影响;以小鼠淋巴细胞为效应细胞,NOD小鼠胰岛细胞瘤NIT细胞为靶细胞,通过乳酸脱氢酶试验研究实验组与对照组细胞毒作用的差异。结果:30只链球菌制剂处理组♀NOD小鼠无发病鼠,而20只生理盐水对照组的糖尿病发病率达55%;组织切片检查发现实验组小鼠β细胞损伤程度减轻;发病的NOD小鼠淋巴细胞对NIT细胞的细胞毒作用明显,与实验组比较差异显著。结论:本实验结果证实A群溶血性链球菌制剂能有效预防NOD小鼠自身免疫性糖尿病的发生,提示该制剂可望成为Ⅰ型糖尿病有效的防治手段之一。
AIM: To investigate the preventive effect of Group A hemolytic streptococcus on diabetic mice with NOD (autoimmune diabetes animal model). Methods: The mice were injected subcutaneously with 0.1mg of 5-day-old ♀NOD mice once a week for 16 weeks to observe their effects on the degree of pancreatic β-cell injury and the incidence of diabetes. Using mouse lymphocytes as effector cells, NOD mouse islet cell tumor NIT cells as target cells, lactate dehydrogenase test to study the difference between the experimental group and the control group cytotoxicity. RESULTS: Thirty streptococcal treatment groups showed no disease in ♀NOD mice, while the incidence of diabetes in 20 saline control groups was 55%. The histopathological examination showed that the damage of β-cell in mice was alleviated and the incidence of NOD was small Mouse lymphocytes on the cytotoxicity of NIT cells significantly, compared with the experimental group significant difference. Conclusion: The results of this experiment confirmed that group A hemolytic streptococcus preparation can effectively prevent the occurrence of autoimmune diabetes in NOD mice, suggesting that the preparation is expected to be an effective prevention and treatment of type I diabetes mellitus.