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目的 :对人群进行HLA DRB的分型及多态性研究 ,发现未曾报道过的HLA DRB等位基因。方法 :用HLA DRB通用引物扩增该个体所有DRB等位基因的第二外显子 ,并对扩增产物进行克隆 ,通过双脱氧法进行DNA测序。结果 :发现一个新的HLA DRB核苷酸序列。结论 :通过与已发表的序列及Genbank已收载的序列比较 ,显示该序列属于HLA DRB4家族的新等位基因 ,我们暂时称它为DRB4 NC。该序列已被Genbank收载 (登录号为AF2 0 75 48)。
OBJECTIVE: To study the typing and polymorphism of HLA DRB in human population and find HLA DRB alleles that have not been reported before. METHODS: The second exon of all DRB alleles of this individual was amplified by HLA DRB universal primers. The amplified products were cloned and sequenced by dideoxy method. Results: A new HLA DRB nucleotide sequence was found. CONCLUSION: This sequence is a novel allele of the HLA DRB4 family by comparison with published sequences and sequences already contained in Genbank, which we tentatively call DRB4 NC. This sequence has been deposited with Genbank (accession number AF2 0 75 48).