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目的:探讨鼻咽癌组织中增殖细胞核抗原(PCNA)、Bcl-2表达情况及与主要临床指标间的关系。方法:以免疫组化检测鼻咽活检组织中PCNA、Bcl-2蛋白表达,并比较与分析两者和鼻咽癌临床分期及颈淋巴结转移的关系。结果:鼻咽部慢性炎性组织中Bcl-2阳性率为9.5％,明显低于鼻咽癌组织中的阳性率(83.1％)(P<0.01)。PCNA、Bcl-2在鼻咽癌中的表达呈明显负相关(r_s=-0.41,P<0.01)。随着临床分期的增高,PCNA增殖指数(PI)分级有增高的趋势,鼻咽癌伴颈淋巴结转移组中PI之Ⅰ级所占比例,较无颈淋巴结转移组下降,而Ⅱ、Ⅲ、Ⅳ级所占比例增加,但无统计学意义。结论:PCNA PI可反映鼻咽癌的增殖状况,可能与预后相关,Bcl-2蛋白可作为鼻咽癌较好的肿瘤标记物,在鼻咽癌的发生上可能起重要作用。
Objective: To investigate the expression of proliferating cell nuclear antigen (PCNA) and Bcl-2 in nasopharyngeal carcinoma (NPC) and their relationship with the main clinical parameters. Methods: The expressions of PCNA and Bcl-2 protein in nasopharyngeal biopsies were detected by immunohistochemistry. The relationship between them and the clinical stages and metastasis of cervical lymph nodes were compared and analyzed. Results: The positive rate of Bcl-2 in nasopharyngeal chronic inflammatory tissues was 9.5%, which was significantly lower than that in nasopharyngeal carcinoma (83.1%) (P <0.01). The expression of PCNA and Bcl-2 in nasopharyngeal carcinoma was negatively correlated (r_s = -0.41, P <0.01). With the increase of clinical stage, the proliferation index (PCI) of PCNA tended to increase. The proportion of grade Ⅰ in NPC with cervical lymph node metastasis was lower than that without cervical lymph node metastasis, while the expression of Ⅱ, Ⅲ, Ⅳ The proportion increased, but not statistically significant. Conclusion: PCNA PI can reflect the proliferation of nasopharyngeal carcinoma, which may be related to the prognosis. Bcl-2 protein may be a good tumor marker for nasopharyngeal carcinoma and may play an important role in the pathogenesis of nasopharyngeal carcinoma.