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目的采用自体血注入法建立兔脑出血模型,应用不同浓度尿激酶干预,观察其对水通道蛋白-4表达水平的影响。方法选用60只成年健康新西兰兔(雌雄不限),采取自体血注入兔脑内囊建立脑出血模型,造模成功后随机分为5组,每组12只,其中A组为对照组,B～E组为不同浓度尿激酶干预组,在造模成功3h后,对照组予以生理盐水干预,其余各组注入不同浓度的尿激酶,检测其AQP-4在脑水肿组织中的表达。结果使用尿激酶干预后,脑出血周围水肿组织AQP-4阳性的表达均显著低于对照组,差异有统计学意义(P<0.01);其中尿激酶浓度为3万U/mL的D组AQP-4的阳性细胞表达水平最低,与其他各浓度组的免疫表达水平比较,差异有统计学意义(P<0.01)。结论脑出血后,尿激酶干预能降低水通道蛋白-4的表达,其中尿激酶浓度为3万U/mL时最明显,而5万U/mL时可以增加兔脑血肿周围AQP-4的表达。
Objective To establish a model of intracerebral hemorrhage in rabbits by autologous blood injection and observe the effects of urokinase on the expression of aquaporin-4 in rabbits. Methods Sixty adult healthy New Zealand rabbits were randomly divided into 5 groups (12 in each group). The rats in group A were control group, B Groups E and E were treated with different concentrations of urokinase. After 3 hours of successful model establishment, the control group was given physiological saline intervention. The rest groups were injected with different concentrations of urokinase to detect the expression of AQP-4 in brain edema. Results After Urokinase intervention, the expression of AQP-4 in edema tissue around cerebral hemorrhage was significantly lower than that in control group (P <0.01). The AQP in group D with Urokinase concentration of 30,000 U / mL -4 expression of the lowest level of the positive cells, and other concentrations of the group compared to the level of expression of the immune, the difference was statistically significant (P <0.01). Conclusions Urokinase can decrease the expression of aquaporin-4 after intracerebral hemorrhage, especially when urokinase is 30 000 U / mL, while it can increase the expression of AQP-4 around rabbit intracerebral hematoma at 50,000 U / mL. .