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我们观察了川芎嗪对环核苷酸磷酸二酯酶活性的影响,以了解川芎嗪引起体内cAMP水平升高的作用机理。我们基本上采用了Mark Chasin等所描述的方法从兔脑的皮质分离提取和纯化环核苷酸磷酸二酯酶。用竞争蛋白结合分析法测定cAMP的
We observed the effects of ligustrazine on the activity of cyclic nucleotide phosphodiesterase in order to understand the mechanism by which tetramethylpyrazine caused the increase of cAMP in vivo. We essentially used the method described by Mark Chasin et al. To extract and purify cyclic nucleotide phosphodiesterase from the cortex of the rabbit brain. CAMP was determined by competitive protein binding assay