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目的构建哮喘气道重塑小鼠模型并观察不同雾化吸入激发时间气道重塑病理学特征的变化。方法采用卵蛋白(ovalbumin,OVA)腹腔致敏小鼠,1%OVA雾化吸入激发小鼠,末次激发24 h后处死小鼠以构建致敏小鼠模型。模型组依据雾化吸入激发时间不同,分为2、4、6、8周和10周5组。收集支气管肺泡灌洗液(bronchoveolar lavage fluid,BALF)分类计数嗜酸性粒细胞总数,肺组织切片HE、PAS、Masson观察病理变化。结果与正常组相比,各激发时长的模型组细胞总数和嗜酸性粒细胞总数均明显增高(P≤0.001),嗜酸性粒细胞浸润,杯状细胞化生均明显加重(P<0.001),但是气道基底膜仅在8周和10周组增厚明显(P≤0.036)。结论气道重塑开始于炎症早期,随着激发时间推移而逐渐加重,但各项气道重塑特征呈现的时间段不一致,气道重塑病理特征的形成需约8周的时间激发才能完整呈现。
Objective To establish a mouse model of airway remodeling in asthma and observe the changes of airway remodeling pathology during different time of inhalation challenge. Methods Mice were sensitized intraperitoneally with ovalbumin (OVA) and challenged with 1% OVA. The mice were sacrificed 24 hours after the last challenge to establish a sensitized mouse model. The model group was divided into 5 groups of 2, 4, 6, 8 and 10 weeks according to different inhalation time of inhalation. The total number of eosinophils was collected by bronchoveolar lavage fluid (BALF). The pathological changes of lung were observed by HE, PAS and Masson. Results Compared with the normal group, the total number of cells and the total number of eosinophils in model group were significantly increased (P≤0.001), eosinophil infiltration and goblet cell metaplasia were significantly increased (P <0.001) However, the airway basement membrane thickened only significantly at weeks 8 and 10 (P≤0.036). Conclusions Airway remodeling begins in the early stage of inflammation and gradually increases with the passage of time. However, the characteristics of various airway remodeling periods are inconsistent, and the formation of airway remodeling pathology takes about 8 weeks to complete Rendered.