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为建立粳稻遗传转化体系,筛选抗性愈伤组织,提高转化植株再生能力,选用羧苄青霉素钠(carbenicillin sodium,Car)、头孢噻肟钠(cefotaxime sodium,Cef)两种抑菌剂和一种标记选择剂潮霉素B(cygromycin B,HyB),分别设4个抗生素浓度处理侵染转化共培养后的粳稻盐稻8号成熟胚愈伤组织。结果表明:Car的抑菌作用强于Cef,Car浓度为500mg/L时抑菌率达到100.0%,抗性愈伤组织的分化率达到42.2%;选择剂HyB浓度为30mg/L可获得86.1%较高质量的抗性愈伤组织,抗性愈伤组织的分化率达到39.5%。结论:在以盐稻8号为受体材料的粳稻遗传转化体系中,抗生素的最佳搭配是500mg/L Car+30mg/L HyB。
In order to establish a genetic transformation system of Japonica rice, screen the resistant callus and improve the regeneration ability of the transformed plants, two antibacterial agents, carbenicillin sodium (Car), cefotaxime sodium (Cef) Cytosporin B (HyB) was selected as the marker of selection. Four antibiotic concentrations were used to treat the callus of Japonica rice. The results showed that the antibacterial activity of Car was stronger than that of Cef, the antibacterial rate reached 100.0% and the resistance rate of callus reached 42.2% when the concentration of Car was 500mg / L, and 86.1% when HyB concentration of 30% Higher quality resistant callus, resistant callus differentiation rate of 39.5%. CONCLUSION: In the japonica rice genetic transformation system using salt rice 8 as the acceptor material, the optimal combination of antibiotics is 500 mg / L Car + 30 mg / L HyB.