荧光聚合酶链反应(PCR)是一相对价廉和敏感的诊断单基因缺陷和/或非整倍体的方法,包括种植前的诊断,但用于分离胎儿红细胞的诊断还较少。因此,作者提出了采用以脐血作为一种从母血中分离胎儿红细胞的一种模式。方法是在产房取10例已分娩的男性胎儿的脐带血。每例10 ml,经Ficoll密度梯度离心和磷酸缓冲液洗涤,获得有核细胞。将细胞离心到玻片上,确保细胞在玻片上很好地分散开,没有重叠。按血液学的BDH快速染色法,用苏木精与伊红对细胞染色,放大200倍下检查染色效果,细胞与胞核很容易 Fluorescent polymerase chain reaction (PCR) is a relatively inexpensive and sensitive method of diagnosing single-gene defects and / or aneuploidy, including pre-implantation diagnostics, but less for the diagnosis of fetal red blood cells. Therefore, the authors propose using cord blood as a model for separating fetal red blood cells from maternal blood. The method is to take the umbilical cord blood of 10 male fetuses who have given birth in the delivery room. Each 10 ml, was washed with Ficoll density gradient centrifugation and phosphate buffer to obtain nucleated cells. Centrifuge the cells on the slide to ensure that the cells are well spread on the slide with no overlap. According to hematology BDH rapid staining method, hematoxylin and eosin staining of cells, magnification 200 times to check the staining effect, cells and nuclei is easy