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为了证实EGF和LIF这两种细胞素与生殖功能的相关性，尤其是与IVF妊娠率的相关性，作者选取25例IVF病例，测定2～3个主卵泡之卵池液中EGF和LIF的含量，比较研究IVF病例中妊娠与未妊娠者与其卵泡泡中EGF和LIF含量的关系。材料与方法：选择IVF病例25例，男性因素和多囊卵巢综合征未包含在内。应用长周期GnRHa／hMG／hCG方案促排卵。病例分A，B两组，A组8例（妊娠）；B组17例（未妊娠）。妊娠以B超下见到孕囊为确证、25例IVF病例，在阴道超声引导下，收集到卵泡液6O份（卵泡液收集后即SOOXg离心10min，-70C保存）。应用酶联免疫吸附法测定卵泡液中EGF和LIF的含量，其敏感度分别为0．2pg／ml和4．Opg／ml；应用化学发光免疫法测定卵泡液中雌二醇（EZ）和孕酮（P）的含量。结果：共测定卵泡液60份。其中A组（妊娠组）18份，B组（未妊娠组）42份。EGF：A组为1．6±1．8pg／ml．B组为9．1±11．6pg／ml；LIF：A组为15．9±9·5pg／ml，B组为165±15．2pg／ml；E2：A组574±245ng／ml，B组为6O1±417ng／ml；P：A组为11．8±5·Opg／ml，B组为11·5±5.3μg／ml；妊娠组EGF的临界值为2pg／ml。上述结果显示：妊娠组之卵泡液中EGF明显低于未妊娠组。而LIF和P，则A，B两组无明显差别。曾有报道，EGF浓度与卵母细胞?
In order to confirm the correlation between EGF and LIF cytokines and reproductive function, especially the IVF pregnancy rate, the authors selected 25 cases of IVF to determine the EGF and LIF in 2 ~ 3 primary follicles Content, a comparative study of IVF cases of pregnant and non-pregnant women and their follicles in the relationship between EGF and LIF content. MATERIALS AND METHODS: Twenty-five cases of IVF were selected. Male factors and polycystic ovary syndrome were not included. Ovulation is induced by long-term GnRHa / hMG / hCG regimen. Cases A, B two groups, A group of 8 cases (pregnancy); B group of 17 cases (not pregnant). Pregnancy to confirm the gestational sac B ultrasound to confirm, 25 cases of IVF cases, under the guidance of vaginal ultrasound collected 6O follicular fluid (follicular fluid collection SOOXg after centrifugation 10min, -70C save). The levels of EGF and LIF in follicular fluid were determined by enzyme-linked immunosorbent assay with sensitivity of 0.2 pg / ml and 4 respectively. Opg / ml; Determination of estradiol (EZ) and progesterone (P) in follicular fluid by chemiluminescence immunoassay. Results: A total of 60 follicular fluid tests. Among them, 18 in group A (pregnancy group) and 42 in group B (non-pregnant group). EGF: A group was 1.6 ± 1.8 pg / ml. Group B was 9.1 ± 11.6 pg / ml; Group A was 15.9 ± 9.5 pg / ml; Group B was 165 ± 15.2 pg / ml; Group A: Group A was 574 ± 245 ng / ml; Group B was 9.1 ± 11.6 pg / Group was 6O1 ± 417ng / ml; P: A group was 11.8 ± 5 · Opg / ml, B group was 11.5 ± 5.3μg / ml; pregnancy group EGF threshold 2pg / ml. The above results show that: the gestational group of follicular fluid was significantly lower than the non-pregnant group of EGF. The LIF and P, then A, B two groups no significant difference. It has been reported that EGF concentration and oocyte?