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为明确~(60)Co-γ对刺梨的辐照诱变效应,采用不同剂量~(60)Co-γ射线(5 Gy,10 Gy,15 Gy,20 Gy和30Gy)辐照处理刺梨幼苗,测定其生长发育及相关生理指标。结果表明:随着辐照剂量的增加,刺梨幼苗成活率降低,10 Gy以上辐照处理幼苗成活率与对照差异极显著,同时幼苗地上部及根系生长发育受到严重抑制;叶片光合色素和淀粉含量随辐照剂量的增加而逐渐降低,且各处理间差异极显著;丙二醛含量随辐照剂量的加大而极显著增加;可溶性糖、可溶性蛋白质、游离脯氨酸含量都随着辐照剂量增加表现为先升后降趋势,并在10 Gy或15 Gy处出现峰值。由此表明,刺梨幼苗适宜辐照剂量在15~20 Gy之间,同时对较低剂量的~(60)Co-γ射线具有一定的耐受能力。研究结果将为刺梨辐射诱变育种提供一定的理论依据。
In order to clarify the mutagenic effects of ~ (60) Co-γ on Radix przewalshimi, radiations of 60 Gy Co-γ rays (5 Gy, 10 Gy, 15 Gy, 20 Gy and 30 Gy) Seedlings, determination of its growth and development and related physiological indicators. The results showed that with the increase of radiation dose, the survival rate of prickly pear seedlings decreased. The survival rate of seedlings irradiated with more than 10 Gy was significantly different from that of the control. At the same time, the growth and development of seedling shoots and roots were severely inhibited. The photosynthetic pigments and starch The contents of soluble sugar, soluble protein and free proline decreased with the increase of irradiation dose, and the differences among treatments were extremely significant. The content of MDA increased significantly with the increase of irradiation dose; According to the increase in dose showed a trend of rising first and then decreasing, and peaked at 10 Gy or 15 Gy. Thus, it is indicated that the optimum dose of Radix przewalskii seedlings is between 15 and 20 Gy, and it is also tolerant to the lower dose of 60Co-γ-rays. The results will provide a theoretical basis for the mutation breeding of Cili pear.