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目的建立过氧化物酶-分光光度法测定小麦粉中过氧化钙含量的方法。方法稀硫酸与样品混合反应,然后用碱液中和,离心分离后得到的上清液,与过氧化物酶溶液、4-氨基安替比林与苯酚的混合磷酸缓冲液水浴显色,于505 nm处测定吸光度。结果本方法回收率96.6%~99.0%,方法检出限和定量限分别为7.4和22.0 mg/kg,线性范围0.4~10μg/m L,r~2为0.9980。结论本方法简单、可靠、准确,适用于小麦粉中过氧化钙含量的测定。
Objective To establish a method for the determination of calcium peroxide content in wheat flour by peroxidase spectrophotometry. Methods The dilute sulfuric acid was mixed with the sample and then neutralized with a lye and the supernatant obtained after centrifugation was developed with a peroxidase solution and a mixed phosphoric acid buffer solution of 4-aminoantipyrine and phenol in a water bath at Absorbance was measured at 505 nm. Results The recoveries of this method ranged from 96.6% to 99.0%. The limits of detection and quantification were 7.4 and 22.0 mg / kg, respectively. The linear range was 0.4-10 μg / m L and r 2 was 0.9980. Conclusion The method is simple, reliable and accurate and suitable for the determination of calcium peroxide content in wheat flour.