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目的分析膜联蛋白A2(Annexin A2)和葡萄糖调节蛋白78(GRP78)分别在人前列腺增生细胞系(BPH-1)、雄激素非依赖性前列腺癌细胞系(LNCaP)和雄激素依赖性前列腺癌细胞系(PC-3)中的差异表达。方法利用双向凝胶电泳结合质谱的蛋白质组学技术,分析Annexin A2和GRP78在三种细胞系的表达;分别采用免疫印迹法和免疫荧光染色法对其进行半定量和定位验证。结果 Annexin A2在BPH-1和LNCaP中低表达,在PC-3细胞中高表达;GRP78在PC-3、BPH-1及LNCaP中表达量逐渐升高(P<0.05)。结论 Annexin A2和GRP78的差异表达可能与前列腺癌发展的不同阶段激素依赖性有关,有望作为前列腺癌诊断和治疗的重要指标。
Objective To investigate the effects of annexin A2 and glucose regulated protein 78 (GRP78) on the proliferation of human prostatic hyperplasia cell line (BPH-1), androgen-independent prostate cancer cell line (LNCaP) and androgen-dependent prostate cancer Line (PC-3). Methods The two-dimensional gel electrophoresis and mass spectrometry proteomics techniques were used to analyze the expression of Annexin A2 and GRP78 in three cell lines. The expression of Annexin A2 and GRP78 was verified by semi-quantitative and immunofluorescent staining. Results Annexin A2 was low expressed in BPH-1 and LNCaP, and highly expressed in PC-3 cells. The expression of GRP78 increased in PC-3, BPH-1 and LNCaP groups (P <0.05). Conclusions The differential expression of Annexin A2 and GRP78 may be related to the hormone dependence at different stages of the development of prostate cancer and is expected to serve as an important indicator for the diagnosis and treatment of prostate cancer.