根据常山碱和异常山碱分子中的喹唑酮能碱水解而形成邻氨基苯甲酸的特点,制订常山植株中碱类含量的比色方法。邻氨基苯甲酸可与经重氮化的N-1-萘基-乙二胺偶合后,于530毫微米处读光密度。此法的灵敏度以光密度值/微克异常山碱/毫升比色液表示为0.111。用此法检查通常用的氯仿萃取法,得知其所回收的常山碱类尚不及实际含量之半。 According to Changshan alkali and abnormal mountain alkali molecules in the hydrolysis of quinazolinone and the formation of anthranilic acid, the characteristics of Changshan plant alkaloids in the development of colorimetric methods. Anthranilic acid may be read at 530 nm after diazotised N-1-naphthyl-ethylenediamine coupling. The sensitivity of this method is expressed as 0.111 as the optical density value / micrograms of abnormal base / mL colorimetric solution. This method is usually used to check the chloroform extraction method, that the recovery of the Changshan alkali is less than half the actual content.