目的研究过氧化氢低温等离子体灭菌效果及影响因素,为科学使用提供参考。方法采用载体定性培养方法,对不同因素影响低温等离子体灭菌器的灭菌效果进行实验室研究。结果在常规的灭菌运行程序条件下,将染菌载体置于孔径1 mm、长度1 000 mm的管腔内,30份枯草杆菌黑色变种芽孢全部达到灭菌,30份嗜热脂肪杆菌芽孢中有23份达到灭菌。在孔径1 mm,长度为150 mm和1 000 mm的管腔内放置嗜热脂肪杆菌芽孢染菌载体,经常规灭菌程序处理,150 mm管腔内30份染菌载体中24个达到灭菌,1 000 mm管腔中只有10个达到灭菌。分别在两端相通和一端盲管的150 mm管腔内各放30份嗜热脂肪杆菌芽孢染菌载体,结果相通管腔内23份达到灭菌,盲管内14个达到灭菌;1 mm孔径管腔内14个达到灭菌,2 mm孔径的管腔内有23个达到灭菌。结论嗜热脂肪杆菌芽孢对过氧化氢低温等离子体抗力强于枯草杆菌黑色变种芽孢;管腔孔径和长度影响低温等离子体灭菌效果。 Objective To study the sterilization effect and influencing factors of hydrogen peroxide plasma at low temperature and provide reference for scientific use. Methods The method of carrier qualitative culture was used to carry out laboratory research on the sterilization effect of different factors on low temperature plasma sterilizer. Results Under the routine conditions of sterilization operation, the dye-carrier was placed in a lumen with a diameter of 1 mm and a length of 1 000 mm. All 30 Bacillus subtilis black spores were sterilized and 30 Bacillus stearothermophilus 23 to sterilize. Placement of the Bacillus stearothermophilus vector in lumens 1 mm in diameter and 150 mm in length and 1 000 mm in length was performed by routine sterilization procedures and 24 of the 30 in 150 mm lumens were sterilized Only 10 out of 1 000 mm lumens were sterilized. 30 Bacillus stearothermophilus vectors were placed in 150 mm lumens which were connected at both ends and one end of the blind tube respectively. The results showed that 23 of them were sterilized in the same lumen and 14 in the blind tube. The diameter of 1 mm Fourteen tubes were sterilized, and 23 tubes with a diameter of 2 mm reached sterility. Conclusions Bacillus stearothermophilus is more resistant to hydrogen peroxide at low temperature plasma than B. subtilis var. Niger. The diameter and length of the lumen affect the sterilization effect of low temperature plasma.