论文部分内容阅读
通过对分布在东海区远海区(济州岛海域)和近海区(长江口以南和以北海域)的三疣梭子蟹(Portunus trituberculatus)自然群体线粒体COⅠ基因部分序列的测定,比较并分析了其群体遗传多样性和遗传结构。在24个采集点共286个样本的线粒体COⅠ序列中共检测到45种单倍型,单倍型多样性为0.864;核苷酸多样性为0.002 84,平均核苷酸变异数是1.686,其中有23个单倍型为单个采集点所独享,Hap3出现频率最高,在23个采样点的83个样本中均有发现,是所有三疣梭子蟹样本的共享中心单倍型。群体遗传多样性分析显示,远海区群体的遗传多样性水平最高,其次为长江口以北群体,长江口以南群体最低。由群体间遗传距离、系统进化树和单倍型网络关系图发现,东海区海域三疣梭子蟹种群间存在基因交流,亲缘关系的远近不以地理位置的远近为依据;AMOVA分子方差分析结果显示,三疣梭子蟹的遗传变异主要来自群体内,而群体间无显著遗传分化,不存在明显的地理趋势。
By analyzing the partial sequence of mitochondrial COI gene in the natural population of Portunus trituberculatus distributed in the sea area off the coast of the East China Sea (Jeju Island) and the offshore area (south of the Changjiang Estuary and north of the Changjiang River), the population of the COⅠ gene was compared and analyzed Genetic diversity and genetic structure. A total of 45 haplotypes were detected in mitochondrial COⅠ sequences of 286 samples at 24 collection sites with a haplotype diversity of 0.864, a nucleotide diversity of 0.002 84, and an average number of nucleotide variations of 1.686, among which Twenty-three haplotypes were unique to a single collection point, and Hap3 was the most frequently found in 83 samples at 23 sampling sites, a shared central haplotype for all Portunus trituberculatus samples. The analysis of population genetic diversity showed that the genetic diversity of far-sea groups was the highest, followed by the populations north of the Yangtze River estuary and the population south of the Yangtze estuary. According to the relationship between population genetic distance, phylogenetic tree and haplotype network, it is found that there is gene exchange among the populations of Portunus trituberculatus in the East China Sea. The distance between the two species is not based on the geographical location. AMOVA analysis of variance shows that, Portunus trituberculatus genetic variation mainly from within the population, but no significant genetic differentiation between populations, there is no obvious geographical trends.