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目的 :评价急性心肌缺血犬模型行激光打孔隧道埋植碱性成纤维细胞生长因子纤维蛋白胶对缺血心肌血管生成及血流灌注的影响。方法 :18只健康杂种犬随机分成三组 ,即单纯冠脉结扎组 (AMI组 )、冠脉结扎加激光打孔组 (TML R组 )和冠脉结扎加激光心肌打孔隧道埋植碱性成纤维细胞生长因子纤维蛋白胶组 (b FGF组 )。所有实验犬均结扎左前降支中段造成急性心肌缺血模型 ,TML R组和 b FGF组在冠脉结扎后 3 0 min于缺血区心肌行激光打孔 (TML R) ,b FGF组并于激光打孔的心肌隧道内埋植碱性成纤维生长因子纤维蛋白胶 (b FGF-FG)。术后 2个月用冠脉血流显像检测缺血区心外膜表面冠脉侧支及心肌内血流 ,用免疫组化 因子相关抗原染色定量缺血心肌内微血管密度及 因子表达。结果 :TML R组及 b FGF组缺血打孔区冠脉侧支及心肌内血流的彩色信号面积及平均吸光度均大于 AMI组 ,以 b FGF组为显著 (P均 <0 .0 1) ,且 b FGF组的上述两项指标高于 TML R组 (P均 <0 .0 5) ;TML R组和 b FGF组缺血打孔区心肌内微血管密度及 因子相关抗原显色面积、平均吸光度均大于 AMI组 (P<0 .0 5或 0 .0 1) ,且 b FGF组三项指标高于 TML R组 (P均 <0 .0 5)。结论 :激光心肌打孔隧道内埋植碱性成纤维细胞生长因子纤维蛋白胶 ,能够进一步促?
OBJECTIVE: To evaluate the effect of bFGF implantation on the angiogenesis and perfusion in ischemic myocardium in a rat model of acute myocardial ischemia. Methods: Eighteen healthy mongrel dogs were randomly divided into three groups: simple coronary artery ligation group (AMI group), coronary artery ligation plus laser drilling group (TML R group) and coronary artery ligation plus laser myocardial perfusion tunnel Fibroblast growth factor fibrin glue (b FGF group). All experimental dogs were ligated with the middle part of the left anterior descending artery to induce acute myocardial ischemia model. TML R and b FGF groups were subjected to laser drilling (TML R) and b FGF group at 30 min after coronary artery ligation Laser-pierced myocardial tunnels harbor fibroblast growth factor fibrin glue (b FGF-FG). Coronary artery blood flow imaging was used to detect the coronary artery branches and myocardial blood flow on the epicardial surface 2 months after operation. The expression of microvessel density and factor in the ischemic myocardium was quantified by immunohistochemical staining. Results: The color signal area and average absorbance of coronary artery branches and intramyocardial blood flow in TML group and b FGF group were greater than those in AMI group and bFGF group (all P <0.01) , And the above two indexes of b FGF group were higher than that of TML R group (all P <0.05); the intramyocardial microvessel density of ischemic perforated area and the area of color of factor related antigen in TML R group and b FGF group were average The absorbance was higher than AMI group (P <0.05 or 0.01), and the three indexes of bFGF group were higher than that of TMLR group (all P <0.05). CONCLUSION: Bovine basic fibroblast growth factor fibrin sealant embedded in the tunnel of laser myocardial perfusion can further promote