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近年来研究证明,T淋巴细胞在体外受特异性抗元或植物血凝素(PHA) 的刺激,可转化为淋巴母细胞,这种体外的淋巴母细胞转化反应往往和体内相应抗元所引起的迟缓型变态反应相符。此外,T淋巴细胞表面有与绵羊红细胞(SRBC) 结合的受体,二者结合后形成玫瑰花瓣样细胞(ERFC) ,这种玫瑰花瓣形成细胞百分率的变化,可用来估测外周血液中T淋巴细胞的水平,因此,淋巴细胞转化试验和玫瑰花瓣反应可作为测定机体细胞免疫状态的指标,目前已用以癌病、免疫缺损病、变态反应性疾病以及器官移植的组织适应性测试和免疫抑制疗法等方面的临床研究。然而,这两种细胞免疫体外测定的方法,试验条件虽不繁杂,但操作技术的标准化,方法的稳定及简化、试验结果的重复性以及可供参考的正常数值等均有待研究统一与解决。为了适应肿瘤临床研究的需要,我们在开展癌肿病人免疫状态测定的过程中,对淋巴细胞转化试验和玫瑰花瓣反应的试验条件作了某些比较研究,以求得到简便而稳定的试验方法。
In recent years, studies have shown that T lymphocytes in vitro by specific anti-lectin or phytohemagglutinin (PHA) stimulation can be transformed into lymphoblasts, this in vitro lymphoblastoid transformation reaction is often caused by the body and the corresponding anti-yuan Consistent with delayed type allergy. In addition, there are receptors on the surface of T lymphocytes that bind to sheep erythrocytes (SRBCs), which combine to form rose-like cells (ERFCs). The percentage change of the percentage of cells forming the rose petals can be used to estimate T lymphocytes in peripheral blood Therefore, the lymphocyte transformation test and the rose petal reaction can be used as indicators for determining the cellular immune status of the body, and have been used to test cancer tolerance, immunodeficiency diseases, allergic diseases, and tissue suitability tests of organ transplantation and immunosuppression Therapy and other aspects of clinical research. However, the two methods of cell immunity assay in vitro, although the test conditions are not complicated, but the standardization of operating techniques, the stability and simplification of the method, the repeatability of the test results and the normal values for reference are to be studied and unified. In order to meet the needs of clinical research on cancer, we conducted some comparative studies on the test conditions of lymphocyte transformation test and rose petal reaction in the course of measuring the immune status of cancer patients in order to obtain a simple and stable test method.