Aim:To investigate the possible association of Q89R,N740N and A1330V poly-morphisms in low-density lipoprotein receptor-related protein 5(LRP5)gene withbone mineral density(BMD)in postmenopausal Chinese women.Methods:Q89R,N740N and A1330V genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)in 647 unrelated healthypostmenopausal Han Chinese women aged 43-76 years in Shanghai.BMD atlumbar spine 1-4 and the left proximal femur including the femoral neck,trochanterand Ward’s triangle were measured by dual-energy X-ray absorptionmetry in allsubjects.Results:The distribution of the Q89R,N740N and A1330V genotypesin this population was as follows:QQ 80.5%,QR 18.7%,and RR 0.8%;TT 66.9%,TC 31.1%,and CC 2.0%;AA 68.0%,AV 29.7%,and VV 2.3%.The frequencies of theQ89R,N740N and A 1330V genotypes and alleles did not deviate from the Hardy-Weinberg equilibrium.We found that the Q89R and A1330V polymorphisms werein linkage disequilibrium in our population(x~2=13.50,P<0.01).Both before and afteradjusting for age,years since menopause,height,and weight,the Q89R or N740Ngenotypes were significantly associated with BMD at the femoral neck(P<0.05).Subjects with the Q89R QQ genotype or the N740N TT genotype had a signifi-cantly higher BMD at the femoral neck,compared with those with the QR/RR orTC/CC genotypes,respectively.No significant association was found betweenA1330V polymorphism and BMD at any site.Conclusion:Our findings suggestthat the LRP5 gene is a candidate for the genetic determination of BMD in post-menopausal Chinese women. Aim: To investigate the possible association of Q89R, N740N and A1330V poly-morphisms in low-density lipoprotein receptor-related protein 5 (LRP5) gene with bone mineral density (BMD) in postmenopausal Chinese women. Methods: Q89R, N740N and A1330V genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 647 unrelated healthypostmenopausal Han Chinese women aged 43-76 years in Shanghai. BMD atlumbar spine 1-4 and the left proximal femur including the femoral neck, trochanterand Ward’s triangle were measured by dual-energy X-ray absorption spectrometry in all objects. Results: The distribution of the Q89R, N740N and A1330V genotypes this population was as follows: QQ 80.5%, QR 18.7%, and RR 0.8%; TT 66.9%, TC 31.1% , and CC 2.0%; AA 68.0%, AV 29.7%, and VV 2.3%. The frequencies of the Q89R, N740N and A 1330V genotypes and alleles did not deviate from the Hardy-Weinberg equilibrium. We found that the Q89R and A1330V polymorphisms were in linkage disequilibrium in our popul Both before and afteradjusting for age, men since menopause, height, and weight, the Q89R or N740Ngenotypes were significantly associated with BMD at the femoral neck (P <0.05). Subjects with the Q89R QQ genotype or the N740N TT genotype had a signifi-cantly higher BMD at the femoral neck, compared with those with the QR / RR orTC / CC genotypes, respectively. Significant association was found betweenA1330V polymorphism and BMD at any site. Conlusion : Our findings suggest that the LRP5 gene is a candidate for the genetic determination of BMD in post-menopausal Chinese women.