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本文研究了不育男性情子中腺苷酸环化酶(AC)活性与精子活率间的关系。不育男性禁欲5天后。在晨8:30—11:30间,手淫将精液直接收集于灭菌容器中。射精后30～120分钟内在光镜下测定精子活率。精子制奋以3ml Harrison洗涤缓冲剂(其中含140mM Nacl,4mM kcl,7mM Mgcl_2,硫酸卡那霉素(1μg/ml)和25mMN—2羟乙基呱嗪—N(?)-2-乙基磺酸)稀释样本,稀释后的精液加3ml10％聚蔗糖并在450Xg离心5分钟,然后在1100Xg离心10分钟,使精子小粒重新悬浮于2ml冷洗涤缓冲液中,呈送分析cAMp浓度和AC活性。
This article studies the relationship between adenylate cyclase (AC) activity and sperm viability in infertile men. Infertility men abstinence after 5 days. Between morning 8: 30-11: 30, masturbation semen is collected directly in a sterile container. Within 30 to 120 minutes after ejaculation, sperm motility was measured under a light microscope. Sperm were stimulated with 3 ml of Harrison wash buffer (containing 140 mM Nacl, 4 mM kcl, 7 mM MgCl 2, kanamycin sulfate (1 μg / ml) and 25 mM N-hydroxyethylpiperazine-N Sulfonic acid), the diluted semen plus 3 ml of 10% Ficoll was centrifuged at 450 × g for 5 minutes and then centrifuged at 1100 × g for 10 minutes to resuspend sperm pellets in 2 ml cold wash buffer for analysis of cAMp concentration and AC activity.