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为探讨甲基丙烯酸环氧丙酯(GMA)诱导细胞转化的机理,选用划痕染料示踪技术(SLDT)初步研究了GMA对人胚肺成纤维细胞间隙连接通讯(GJIC)的影响。用0.5、2.5和5.0mg/L的GMA给细胞染毒12h,经划痕导入荧光黄染料并测定各组细胞中该荧光染料向其相邻细胞扩散的程度。结果表明,在染毒剂量及时间范围内,GMA对人胚肺成纤维细胞GJIC产生较强的抑制作用,呈良好的剂量-效应关系。其中,2.5和5.0mg/L剂量组细胞的GJIC明显降低。结果提示GMA对细胞间隙通讯的抑制作用可能是其导致细胞恶性转化的重要机制之一。
To investigate the mechanism of GMA-induced cell transformation, Scratch Dye Tracer (SLDT) was used to study the effects of GMA on GJIC in human embryonic lung fibroblasts. The cells were exposed to 0.5, 2.5, and 5.0 mg/L GMA for 12 hours. The fluorescent yellow dye was introduced into the scratches and the degree of diffusion of the fluorescent dye to its neighboring cells in each group of cells was determined. The results showed that GMA exerted a strong inhibitory effect on GJIC of human embryonic lung fibroblasts within a dose and time range of exposure, showing a good dose-effect relationship. Among them, the GJIC of cells at doses of 2.5 and 5.0 mg/L was significantly decreased. The results suggest that the inhibitory effect of GMA on intercellular communication may be one of the important mechanisms leading to the malignant transformation of cells.