Objective: To elucidate the effect of age and sexual abstinence on semen quality (semen volume, total count, progressive motility, vitality and morphology). Methods:A total of 730 semen samples were analyzed. Subjects were grouped according to the age (20-29, 30-34, 35-39 and 40-50) and abstinence (2-3, 4-5 and 6-7). Semen parameters were evaluated following WHO standard criteria. Results: Analysis of 730 semen samples showed negative correlation of progressive motility (r=-0.131, P< 0.01), vitality (r=-0.173, P< 0.01), morphology (r=-0.324, P< 0.01) with age. With increase in age percentage of progressive motility, vitality and normal morphology in mean values declined after the age group of 35-39 to 40-50 years, but no change in volume and count were observed. Increase in abstinence with individual days significantly affected semen volume (H=20.65, P<0.001), count (H=36.67, P<0.01), progressive motility (H=13.53, P<0.05) and vitality (H=15.33, P<0.01). But, no effect was found on sperm morphology. Mann Whitney U test confirmed the changes in semen volume, total count and vitality in paired grouping from 2-7 days (P<0.05), but changes in sperm motility were observed after 5 days of abstinence in each paired group upto 7 days (P<0.05). Mean values of semen parameters among three abstinence groups (2-3, 4-5 and 6-7 days) also showed similar result. Conclusions:In the present study, age negatively affected progressive motility, vitality and morphology of human sperm. Semen samples showed intra varied results within WHO amended abstinence period.