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目的 观察羟基喜树碱 (hydroxycom ptothecine,HCPT)联合 Ver对实验性增殖性玻璃体视网膜病变 (proliferative vitreoretinopathy,PVR)的防治作用。方法 以原代培养成纤维细胞 (fibroblast,FB)每眼 10 0 0 0 0个经兔眼睫状体扁平部注射到玻璃体腔内 ,制作成兔 PVR模型 ,共 12只兔 12眼。随机分成药物治疗组和生理盐水对照组 (每组 6只 ) ,治疗组以 5 m g· L- 1 HCPT联合 5 mg· L- 1 Ver进行每 3天 1次共 15 d的玻璃体腔注射治疗。对照组以同法注入生理盐水。以裂隙灯显微镜、直接检眼镜、螺旋 CT进行随访观察。结果 药物治疗组无论在玻璃体混浊程度进展上、在视网膜血管扩张程度上以及牵拉性视网膜脱离 (tractional retinal detachment,TRD)发生发展上都较对照组缓和。进行此浓度药物治疗后未见任何可见的眼部毒副作用。螺旋 CT可以监测到治疗组 PVR较对照组PVR在增殖膜密度上差异有显著性。结论 HCPT联合 Ver经玻璃体腔内注射途径可以有效防治实验性兔眼 PVR。螺旋 CT可对兔眼 PVR进行定性、定量分析
Objective To observe the preventive and therapeutic effects of hydroxycamptothecin (HCPT) combined with Ver on experimental proliferative vitreoretinopathy (PVR). Methods Primary rabbit fibroblasts (FBs) were injected into the vitreous cavity through the flattened part of the ciliary body of the ciliary body with 100 000 eyes per each eye to make a rabbit model of PVR, 12 eyes in 12 rabbits. The rats in the treatment group were treated with 5 mg · L -1 HCPT plus 5 mg · L -1 Ver once for every 15 days for 15 days after intravitreal injection. Control group with normal saline. Slit lamp microscopy, direct ophthalmoscopy, spiral CT were followed up. Results Compared with the control group, the drug-treated group showed no significant difference in the degree of vitreous opacity and retinal vasodilatation and the development of traction retinal detachment (TRD). After this concentration of drug treatment did not see any visible ocular side effects. Spiral CT can be observed in the treatment group PVR compared with the control group PVR proliferative membrane density differences were significant. Conclusion HCPT combined with Ver via intravitreal injection can effectively prevent and treat experimental rabbit PVR. Spiral CT can be qualitative and quantitative analysis of rabbit eyes PVR