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用单极1.0mA的直流电刺激大鼠前脑基底核的方法制作类痴呆动物模型,手术后第4天喂以生地、熟地、菖蒲、远志等为主的补肾、调心方剂及喜得镇等药17天。在第21天取材,恒冷箱切片,进行乙酰胆碱酯酶(AchE)组织化学反应。观察基底核苍白球大神经原的反应变化。实验结果为模型组的大细胞神经原核团面积显著变小,AchE阳性反应的细胞及突起等数量显著减少,AchE反应显著降低。调心及补肾组的核团面积都比模型组大,阳性细胞数量及突起分枝也都较多,AchE反应比模型组明显提高。实验结果表明应用调心、补肾中药及喜得镇动物的大细胞神经原核团面积、细胞及突起数量、AchE反应都有明显的提高。证实此类中药可能有调节脑皮层活动的效应。
An animal model of dementia was made using a unipolar 1.0 mA direct current to stimulate the basal nucleus of rat forebrain. After the fourth day of surgery, the kidneys, heart-regulating formulae, and root recipes, such as Rehmannia, Rehmannia lanceolata, Rhizoma Medicine for 17 days. On the 21st day, samples were obtained from cryostat boxes and histochemical reactions of acetylcholinesterase (AchE) were performed. The basal ganglia globus pallidus was observed for changes in its response. The experimental results showed that the area of large cell neuronal pronucleus in the model group was significantly smaller, the number of cells and processes of AchE positive reaction was significantly reduced, and the AchE response was significantly reduced. The core area of the self-aligning and kidney-tonifying groups was larger than that of the model group, and the number of positive cells and neurite outgrowth were also higher, and the AchE response was significantly higher than that of the model group. The experimental results showed that the area of large cell neuronal pronucleus, the number of cells and processes, and the AchE response were significantly improved in the animals of Tiaoxin, Bushen Chinese medicine and Xideng town. It was confirmed that such Chinese medicine may have the effect of regulating cerebral cortical activity.