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目的观察胃癌组织和细胞中高尔基体转运蛋白P115和MIF的表达情况,探讨其在胃癌发生、发展中的意义。方法用S-P、Western blot和RT-PCR法检测P115及MIF在正常胃黏膜和胃癌组织,3种细胞株(正常胃黏膜上皮GES-1,不同分化程度的胃癌细胞株MKN-28,BGC-823)中的蛋白和mRNA表达情况。结果 P115和MIF在胃癌组织中的阳性表达率分别为73.3%、80%,在正常胃黏膜中的阳性表达率分别为40%、46.7%,胃癌组织中P115和MIF的表达明显高于正常胃黏膜(P<0.01),且P115的表达和MIF的表达呈正相关(r=0.433,P=0.017);胃癌组织中P115、MIF的蛋白和mRNA表达水平明显高于正常胃黏膜组织(P<0.01)。免疫细胞化学,Western blot和RT-PCR结果显示:P115、MIF在胃癌细胞株MKN-28、BGC-823中的蛋白及mRNA表达水平明显高于正常胃黏膜上皮GES-1(P<0.01);且低分化胃癌细胞株BGC-823中P115和MIF的表达水平明显高于高分化胃癌细胞株MKN-28(P<0.05)。结论 P115和MIF的过表达,可能在胃癌的发生过程中起协同作用;对P115和MIF相互作用机制的深入探讨有可能使其成为研究胃癌发生、发展的分子机制的新靶点。
Objective To observe the expression of Golgi transporter P115 and MIF in gastric cancer tissues and cells and to explore its significance in the occurrence and development of gastric cancer. Methods The expressions of P115 and MIF in normal gastric mucosa and gastric cancer tissues, three kinds of cell lines (normal gastric mucosa epithelial cells GES-1, different differentiation gastric cancer cell lines MKN-28, BGC-823 ) In the protein and mRNA expression. Results The positive rates of P115 and MIF in gastric cancer tissues were 73.3% and 80%, respectively. The positive rates of P115 and MIF in normal gastric mucosa were 40% and 46.7% respectively. The expressions of P115 and MIF in gastric cancer tissues were significantly higher than those in normal gastric mucosa (P <0.01). The expression of P115 was positively correlated with the expression of MIF (r = 0.433, P = 0.017). The protein and mRNA expressions of P115 and MIF in gastric cancer were significantly higher than those in normal gastric mucosa (P <0.01) ). The results of immunocytochemistry, Western blot and RT-PCR showed that the expression of P115 and MIF in gastric cancer cell lines MKN-28 and BGC-823 was significantly higher than that in normal gastric epithelium GES-1 (P <0.01). The expression of P115 and MIF in poorly differentiated gastric cancer cell line BGC-823 was significantly higher than that in well-differentiated gastric cancer cell line MKN-28 (P <0.05). Conclusion The overexpression of P115 and MIF may play synergistic roles in the pathogenesis of gastric cancer. Further investigation of the interaction between P115 and MIF may make it a new target for studying the molecular mechanism of the occurrence and development of gastric cancer.