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目的观察哮喘患儿血清可溶性细胞间粘附分子(sICAM1)和白介素8(IL8)质量浓度及其相关性。方法随机选择2003年10月至2004年6月温州医学院育英儿童医院收治的哮喘患儿急性发作期30例、缓解期30例,30名健康儿童为正常对照组,采用酶联免疫吸附试验(ELISA)测定3组血清sICAM1和IL8质量浓度,并分析其相关关系。结果急性发作组sICAM1为(268.56±24.76)μg/L,IL8为(38.06±8.77)μg/L;缓解期sICAM1为(204.90±25.00)μg/L,IL8为(8.85±1.47)μg/L;正常对照组sICAM1为(186.49±16.55)μg/L,IL8为(7.56±1.68)μg/L。sICAM1的组间差异显著(F=97.50,P<0.01);IL8的组间差异显著(H=64.97,P<0.01)。sICAM1与IL8呈正相关,相关系数r为0.80,P<0.01。结论儿童哮喘急性期sICAM1、IL8明显升高,二者呈正相关,在哮喘急性期炎症反应中sICAM1与IL8起相互协同和促进作用,是儿童哮喘急性发作期炎症反应发生的机制之一。
Objective To observe the serum concentrations of soluble intercellular adhesion molecule (sICAM1) and interleukin 8 (IL8) in children with asthma and their correlation. Methods Thirty patients with acute asthma were randomly selected from Yuyin Children’s Hospital of Wenzhou Medical College from October 2003 to June 2004. Thirty patients in remission and 30 healthy children were selected as normal control group by enzyme-linked immunosorbent assay (ELISA) ELISA) were used to measure the serum concentrations of sICAM1 and IL8 in three groups and to analyze their correlation. Results The sICAM1 level in the acute attack group was (268.56 ± 24.76) μg / L and that of IL8 was (38.06 ± 8.77) μg / L and that of sICAM1 was (204.90 ± 25.00) μg / L and (8.85 ± 1.47) μg / L, The normal control group sICAM1 (186.49 ± 16.55) μg / L, IL8 (7.56 ± 1.68) μg / L. There was significant difference between the groups of sICAM1 (F = 97.50, P <0.01). There was significant difference between groups (H = 64.97, P <0.01). sICAM1 was positively correlated with IL8, the correlation coefficient r was 0.80, P <0.01. Conclusions The levels of sICAM1 and IL8 are significantly increased in children with acute asthma, and they are positively correlated with each other. In the acute phase of asthma, sICAM1 and IL8 play a synergistic role in promoting the development of childhood asthma.