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DNA分子标记是继形态标记、细胞学标记、生化标记之后发展起来的一种新的更为理想的遗传标记,其在中药研究中发挥了重要作用。DNA分子标记包括以分子杂交(southern杂交)为基础的DNA分子标记技术、以PCR为基础的DNA分子标记技术和以DNA序列为基础的分子标记技术。本文对DNA分子标记的分类、原理及其在中药研究中的应用进行了综述。
DNA molecular marker is a new and more ideal genetic marker developed after morphological marker, cytological marker and biochemical marker, which play an important role in the study of traditional Chinese medicine. DNA molecular markers include DNA molecular markers based on molecular hybridization (southern hybridization), PCR-based DNA molecular markers and DNA sequence-based molecular markers. This article reviews the classification, principle and application of DNA molecular markers in the study of Chinese medicine.