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我们于1991年对市区17个单位的114台电话污染情况进行了调查。结果如下。调查对象医院3所,宾馆旅社2个,公用电话7处,机关单位5个。内容与方法 (1)按卫生部“消毒技术规范”,用浸有无菌生理盐水的灭菌棉拭子,分别在电话的受话器和把柄上往返涂抹10次后,放于10ml灭菌生理盐水中进行活菌计数。(2)取上液0.1ml涂于血琼脂平板上,进行乙型链球菌检验。(3)以同样的采样方法将样品放于胆盐乳糖肉汤培养基,置37℃培养24h(?)后接种SS琼脂平板进行沙门氏菌检验。(4)胆盐乳糖肉汤产酸产气者接种麦康凯琼脂平板,进行大肠菌检验。(5)活菌计数的剩余样品经透析袋浓缩后,用固相放射免疫(SPRIA)法检测HBsAg。(6)试验药
In 1991, we investigated 114 telephone pollution cases in 17 units in urban areas. The result is as follows. Three hospitals were surveyed, two hotels and guesthouses, seven public telephones and five institutional units. Content and methods (1) According to the Ministry of Health “Disinfection Technical Code”, with a sterile cotton swab immersed in sterile saline, respectively, the phone receiver and handle back and forth 10 times, placed in 10ml sterile saline Live bacteria count. (2) Take the liquid 0.1ml coated on blood agar plate for Streptococcus B test. (3) Samples were placed in bile salt lactose broth medium by the same sampling method, and then plated on SS agar plate for Salmonella test at 37 ℃ for 24h (?). (4) bile salt lactose broth acid production gas were inoculated MacConkey agar plate, coliform bacteria test. (5) The remaining samples of viable count were concentrated in the dialysis bag and tested for HBsAg by solid-phase radioimmunoassay (SPRIA). (6) test drug