论文部分内容阅读
裂殖子表面蛋白1(MSP-1)已成为红细胞期疟原虫疫苗研究的热点。作者报道了用约氏疟原虫MSP-1(PyMSP-1)编码的DNA疫苗免疫小鼠,可获得抗子孢子攻击感染的免疫反应,效果与同类编码抗原加佐剂相似,免疫后的小鼠显示高水平的保护性抗体。 PyMSP-1的构建:VR1012载体插入一个新的多接头以连接小基因组件和人体组织纤溶酶原激活物(tPA)的特异信号序列;另一载体VR 1012 tPA p2 p30,含破伤风毒素p2和p30辅助性T细胞表位,从约氏疟原虫
Merozoite surface protein 1 (MSP-1) has become a hotspot in red blood cell plasmodium vaccine research. The authors reported that immunization of mice with a DNA vaccine encoded by Plasmodium yoelii MSP-1 (PyMSP-1) yielded an immune response against infection by sporozoites that resembled that of similar coding antigens plus adjuvants and that immunized mice showed High levels of protective antibodies. Construction of PyMSP-1: The VR1012 vector was inserted into a new polylinker to link the specific signal sequences of the minigenome and human tissue plasminogen activator (tPA); another vector, VR 1012 tPA p2 p30, containing tetanus toxin p2 And p30 helper T cell epitopes from Plasmodium yoelii