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目的探讨HPV16型病毒感染与宿主人类白细胞抗原HLA-DQB1基因多态性和喀什维吾尔族宫颈癌发生的相关性。方法采用导流杂交基因芯片技术及聚合酶联反应序列特异性寡核苷酸探针(Polymerase chain reaction sequence-specific oligo-nucleotide,PCR-SSO)法检测111例喀什维吾尔族宫颈癌患者、100例正常妇女宫颈组织HPV感染及型别分布和HLA-DQB1的等位基因。结果 (1)111例宫颈癌患者中,HPV总感染率81.9%(91/111),其中HPV16型感染占的比例最高,为91.2%(83/91),100例正常宫颈组织中HPV阳性者为9例,阳性率为9.00%(9/100),其中HPV16阳性2例,两组之间差异有显著性(P<0.01)。(2)HLA-DQB1*03在宫颈癌组中出现的频率明显低于对照组,两组之间差异有统计学意义(χ2=4,P=0,OR=0.607,95%CI=1.042~2.478)。HLA-DQB1*06在宫颈癌组中出现的频率明显高于对照组,两组之间差异有统计学意义(χ2=7.112、P=0,OR=2,95%CI=1.204~3.549)。宫颈癌中HLA-DQB1*06阳性者HPV16的感染率明显高于阴性者(χ2=4.907,P=0.027,OR=2,95%CI=1.092~6.840),两组比较差异有统计学意义,说明携带HLA-DQB1*06等位基因的维吾尔族妇女更容易被HPV16感染。HLA-DQB1的其他等位基因在宫颈癌组及对照组中出现的频率差异无显著性(P>0.05)。结论 HLA-DQB1*03可能为维吾尔族妇女宫颈癌的保护基因,而HLA-DQB1*06可能与维吾尔族妇女对宫颈癌的遗传易感性有关,是维吾尔族妇女对宫颈癌的易感基因,HLA-DQB1*06基因型可能增加了HPV16阳性妇女患宫颈癌的危险性,可能是维吾尔族妇女宫颈癌患病率高的原因之一。维吾尔族妇女宫颈癌易感基因及保护基因的检测可能在高危人群的检测及群体遗传干预工作中有一定的指导意义。
Objective To investigate the association of HPV16 infection with HLA-DQB1 polymorphism of host human leukocyte antigen (HLA-DQB1) and cervical cancer in Kashgar. Methods 111 cases of Kashgar Uigur patients with cervical cancer were examined by flow cytometry and polymerase chain reaction sequence-specific oligo-polymerase chain reaction (PCR-SSO) HPV infection in normal women and their genotype distribution and HLA-DQB1 alleles. Results (1) Among 111 cases of cervical cancer, the total infection rate of HPV was 81.9% (91/111), of which HPV16 infection accounted for the highest proportion of 91.2% (83/91), 100 cases of normal cervical tissue HPV-positive The positive rate was 9.00% (9/100), of which HPV16 was positive in 2 cases. The difference between the two groups was significant (P <0.01). (2) The frequency of HLA-DQB1 * 03 in cervical cancer group was significantly lower than that in control group (χ2 = 4, P = 0, OR = 0.607, 95% CI = 1.042 ~ 2.478). The frequency of HLA-DQB1 * 06 in cervical cancer group was significantly higher than that in control group (χ2 = 7.112, P = 0, OR = 2, 95% CI = 1.204-3.549). The infection rate of HPV16 in HLA-DQB1 * 06 positive cases of cervical cancer was significantly higher than that of negative ones (χ2 = 4.907, P = 0.027, OR = 2, 95% CI = 1.092-6.840) Indicating that Uighur women carrying the HLA-DQB1 * 06 allele are more likely to be infected with HPV16. There were no significant differences in the frequencies of other HLA-DQB1 alleles between cervical cancer group and control group (P> 0.05). Conclusion HLA-DQB1 * 03 may be the protective factor of cervical cancer in Uyghur women, while HLA-DQB1 * 06 may be related to the genetic susceptibility of Uighur women to cervical cancer. It is a common risk factor of cervical cancer in Uighur women. HLA- The -DQB1 * 06 genotype may increase the risk of cervical cancer in HPV16-positive women, which may be one of the reasons for the high prevalence of cervical cancer in Uigur women. The detection of cervical cancer susceptibility genes and protective genes in Uygur women may be of guiding significance in the detection of high-risk groups and population genetic intervention.