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一氧化氮(NO)是调节神经、心血管、内分泌和免疫系统非常重要的细胞信使分子.NO的研究不仅可以阐明许多生理现象,而且可能为许多疾病的防治提供新的途径和手段.但是,对生物体系NO的检测,目前所采用的方法均有一定的缺陷,如灵敏度不高、特异性不强等.因此,建立一种快速、简便、灵敏和高专一性的可定量分析生物体系中NO的方法十分重要.Joseph和Hans-Gert等人曾用氮氧自由基和环状化合物与NO作用检测NO,但因反应速度太慢和只有6％的捕捉效率未能付诸应用,我们根据有机化合物结构理论,设计、合成了一系列的咪唑类NO自旋探针Ⅰ,利用电子自旋共振(ESR)波谱方法考察了自旋探针的取代基效应,初步建立了一套测量微量NO的方法.目前,可检测到5×10~(-12)摩尔的NO,而且有很强的专一性,不受超氧自由基和羟基自由基的干扰,亦不受介质的pH和盐浓度的影响.
Nitric oxide (NO) is a cell messenger that regulates the nervous, cardiovascular, endocrine and immune systems.NO studies not only shed light on many physiological phenomena but may also provide new avenues and tools for the prevention and treatment of many diseases.However, The detection of NO in biological system, the methods used at present have some defects, such as low sensitivity, low specificity, etc. Therefore, to establish a fast, simple, sensitive and high specificity of quantitative biological systems The method of NO is very important.Joseph and Hans-Gert et al. Used nitrogen and oxygen free radicals and cyclic compounds to detect NO with NO, but because the reaction was too slow and only 6% of the capture efficiency could not be applied, Based on the structure theory of organic compounds, a series of imidazole NO spin probes Ⅰ were designed and synthesized. The substituent effects of spin probes were investigated by electron spin resonance (ESR) spectroscopy. A set of micro - NO method.Currently, can detect 5 × 10 ~ (-12) mole of NO, and has a strong specificity, free from superoxide radicals and hydroxyl radicals interference, is not affected by the media pH and Effect of salt concentration.