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目的了解广西南宁某警犬养殖训练基地蜱虫及病原体携带情况。方法 2013年7月在广西南宁某警犬养殖训练基地,采用逆毛式检蜱法采集犬体表以及犬舍墙壁的蜱虫,用巴贝虫属18S rRNA通用引物的巢式PCR、原核生物16S rRNA及真核生物线粒体16S rRNA通用引物的PCR和序列测定方法,鉴定蜱虫体内的病原体感染情况和蜱虫种类。结果从警犬体表及犬舍墙壁共计采集5只饱血蜱和13只饥饿蜱;经PCR鉴定均为血红扇头蜱(Rhipicephalus sanguineus)。蜱虫体内扩增到田鼠巴贝虫(Babesia microti)、伯纳特氏立克次氏体(Coxiella burnetii)、假单胞球菌(Pseudomonas sp.)、甲基杆菌(Methylobacterium sp.)DNA序列,阳性率分别为27.8%(5/18)、22.2%(4/18)、11.1%(2/18)、11.1%(2/18)。结论南宁某警犬养殖训练基地蜱虫存在一定比例的田鼠巴贝虫、伯纳特氏立克次氏体、假单胞球菌、甲基杆菌感染阳性率,对接触人员、及其他牲畜有潜在感染的风险,应加强预防和控制。
Objective To understand the situation of ticks and pathogens in a police dog breed training base in Nanning, Guangxi. Methods In July 2013 in Nanning, Guangxi, a police dog breed training base, the use of inverted wool tick tick method to collect the dog body wall and table tick ticks, using Babesia 18S rRNA universal primer nested PCR, prokaryotic 16S rRNA and eukaryotic mitochondrial 16S rRNA universal primer PCR and sequence determination methods to identify the ticks in vivo pathogen infection and tick species. Results A total of 5 satyronis and 13 hungry ticks were collected from the dogs body surface and wall of the kennel. The Rhipicephalus sanguineus were identified by PCR. The ticks were expanded in vivo to Babesia microti, Coxiella burnetii, Pseudomonas sp., Methylobacterium sp. DNA sequences, The positive rates were 27.8% (5/18), 22.2% (4/18), 11.1% (2/18) and 11.1% (2/18) respectively. Conclusions There is a certain proportion of ticks in Baboon, Laburnum, Bernard’s rickettsia, Pseudomonas and Methytacillosis positive in ticks of a police dog breed training base in Nanning. Potential infection to contact personnel and other livestock The risk should be strengthened to prevent and control.