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目的探讨砷对大鼠胰岛β细胞(INS-1)p53、mdm2和Kras基因和蛋白表达的影响。方法荧光实时定量PCR法检测亚砷酸钠对大鼠胰岛β细胞中野生型p53(Tp53)、mdm2和Kras基因表达的影响,Western blot检测亚砷酸钠对大鼠胰岛β细胞突变型p53和mdm2蛋白表达的影响。结果亚砷酸钠作用于INS-1细胞后,Tp53基因水平降低,mdm2、Kras基因水平升高;突变型p53和mdm2蛋白表达增加。结论亚砷酸钠可使INS-1细胞中抑癌基因Tp53向突变型p53转变,癌基因mdm2和Kras的水平升高。
Objective To investigate the effects of arsenic on gene and protein expression of p53, mdm2 and Kras in rat pancreatic β-cells (INS-1). Methods The expression of wild type p53 (Tp53), mdm2 and Kras in rat pancreatic β cells was detected by real-time fluorescence quantitative PCR. Western blot was used to detect the effect of arsenite on islet β-cell mutant p53 and Effect of mdm2 protein expression. Results After treated with sodium arsenite, the levels of Tp53 gene and mdm2 gene and Kras gene were increased in INS-1 cells, and the expression of mutant p53 and mdm2 protein increased. Conclusion Sodium arsenite can induce the transformation of tumor suppressor gene Tp53 to mutant p53 in INS-1 cells, and increase the levels of oncogenes mdm2 and Kras.